PCR Fluorescence Analysis Case

Real-time PCR (quantitative PCR, qPCR) instruments demand high-precision optical LED sources capable of delivering accurate fluorescence excitation and detection across multiple genetic testing and molecular diagnostic channels. In the field of genetic testing, pathogen detection, and oncology biomarker screening, qPCR sensitivity directly depends on the spectral purity, luminous intensity stability, and rapid switching performance of the LED excitation source. Common challenges include wavelength drift caused by temperature fluctuations, insufficient luminous intensity leading to weak signal acquisition, and package thermal resistance that limits continuous high-throughput operation.

Queendom LED Solution

Queendom provides a full lineup of biomedical optical LEDs specifically engineered for real-time PCR fluorescence analysis systems. Our LED sources are optimized to match mainstream fluorescence channels with precise peak-wavelength control and high coupling efficiency.

Package Options:

• DIP 5mm / 8mm / 10mm: High directionality, easy collimation
• SMD 3535 high-power: High brightness, low thermal resistance
• SMD 5050 multi-chip: Multi-channel parallel excitation

Key Advantages:

• High coupling efficiency: ±8° (DIP), ±12° (SMD)
• Rapid thermal management: Junction temp < 85°C
• Spectral consistency: ±2 nm batch deviation
• Instantaneous switching: Microsecond-level ON/OFF

Biomedical LEDs — SMD 3535 / 5050 for PCR fluorescence excitation

Wavelength Configuration

Technical Parameters

• Spectral half-width (FWHM): ≤ 20 nm (typical 15 nm)
• Luminous intensity stability: ±2% over 8-hour continuous operation
• Thermal resistance (RθJA): ≤ 8 K/W for SMD 3535
• Operating current: 20–350 mA (DIP/SMD 3535), 60–700 mA (SMD 5050)
• Lifespan (L70): ≥ 50,000 hours
• RoHS / REACH: Fully compliant

Validation & Certification

• Biocompatibility: ISO 10993-5 cytotoxicity testing passed
• EMC compliance: IEC 61326-1 for laboratory equipment
• Traceability: Full lot-code from epitaxial wafer to packaged device

Related Links

Solution: Biomedical LEDs Solutions
Product: SMD LED Products | LED Diodes & Chips
Case Studies: PCR LED Parameters Guide | Medical LED Application | PCR Laboratory Lighting